11. Sturgeon

CM,

Ditadi

A,

Awong

G,

Kennedy M, Keller G (2014) Wnt signaling

controls the specification of definitive and

primitive hematopoiesis from human pluripo-

tent stem cells. Nat Biotechnol 32(6):554–561

12. Wang Y, Gao J, Wang H, Wang M, Wen Y,

Guo J, Su P, Shi L, Zhou W, Zhou J (2019)

R-spondin2 promotes hematopoietic differen-

tiation of human pluripotent stem cells by acti-

vating TGF beta signaling. Stem Cell Res Ther

10(1):136

13. Ran D, Shia WJ, Lo MC, Fan JB, Knorr DA,

Ferrell PI, Ye Z, Yan M, Cheng L, Kaufman DS

et al (2013) RUNX1a enhances hematopoietic

lineage commitment from human embryonic

stem cells and inducible pluripotent stem

cells. Blood 121(15):2882–2890

14. Ramos-Mejia

V,

Navarro-Montero

O,

Ayllon V, Bueno C, Romero T, Real PJ,

Menendez P (2014) HOXA9 promotes hema-

topoietic commitment of human embryonic

stem cells. Blood 124(20):3065–3075

15. Wang H, Wang M, Wang Y, Wen Y, Chen X,

Wu D, Su P, Zhou W, Shi L, Zhou J (2020)

MSX2

suppression

through

inhibition

of

TGFbeta signaling enhances hematopoietic

differentiation

of

human

embryonic

stem

cells. Stem Cell Res Ther 11(1):147

16. North TE, Goessling W, Peeters M, Li PL,

Ceol C, Lord AM, Weber GJ, Harris J, Cutting

CC, Huang P et al (2009) Hematopoietic stem

cell development is dependent on blood flow.

Cell 137(4):736–748

17. Diaz MF, Li N, Lee HJ, Adamo L, Evans SM,

Willey HE, Arora N, Torisawa YS, Vickers DA,

Morris SA et al (2015) Biomechanical forces

promote blood development through prosta-

glandin E2 and the cAMP-PKA signaling axis. J

Exp Med 212(5):665–680

18. Kwan W, Cortes M, Frost I, Esain V, Theodore

LN, Liu SY, Budrow N, Goessling W, North

TE (2016) The central nervous system regu-

lates embryonic HSPC production via stress-

responsive glucocorticoid receptor signaling.

Cell Stem Cell 19(3):370–382

19. Bourgine

PE,

Klein

T,

Paczulla

AM,

Shimizu T, Kunz L, Kokkaliaris KD, Coutu

DL, Lengerke C, Skoda R, Schroeder T et al

(2018) In vitro biomimetic engineering of a

human hematopoietic niche with functional

properties. Proc Natl Acad Sci U S A 115

(25):E5688–E5695

20. Lundin V, Sugden WW, Theodore LN, Sousa

PM, Han A, Chou S, Wrighton PJ, Cox AG,

Ingber DE, Goessling W et al (2020) YAP

regulates hematopoietic stem cell formation in

response to the biomechanical forces of blood

flow. Dev Cell 52(4):446–460. e445

21. Yang Y, Liu C, Lei X, Wang H, Su P, Ru Y,

Ruan X, Duan E, Feng S, Han M et al (2016)

Integrated biophysical and biochemical signals

augment Megakaryopoiesis and Thrombopoi-

esis in a three-dimensional rotary culture sys-

tem. Stem Cells Transl Med 5(2):175–185

22. Cao

X,

Yakala

GK,

van

den

Hil

FE,

Cochrane

A,

Mummery

CL,

Orlova

VV

(2019) Differentiation and functional compar-

ison of monocytes and macrophages from

hiPSCs

with

peripheral

blood

derivatives.

Stem Cell Rep 12(6):1282–1297

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Xiaohua Lei et al.